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These reactions are generally a consequence of the aggregation of IgE-occupied high-affinity receptors for IgE (FcRIs), which is induced by multivalent antigen

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Circular dichroism Peptides at concentration of 1 mg/ml, diluted in 10 mM HEPES pH 7.0 or PBS, without or with 2,2,2-trifluoroethanol (TFE, Sigma-Aldrich, St Louis, MO, United States) at concentrations in the range of 525% (commonly used to stabilize secondary structure of polypeptides), and diluted in 10 mM HEPES pH 7.0 with 30 mM SDS (BioShop Canada Inc., Burlington, Canada) (to mimic anionic environment) were prepared

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