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Expression of cyclooxygenase-2 in human epithelial skin lesions: a systematic review of immunohistochemical studies

Psychiatry 31, 283285

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]

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Finally, VEGF secreted in the co-culture system may get bound to the extracellular matrix, where it may not be accessible to VEGF antibodies, but may still be able to be affected by receptor inhibition