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The resulting supernatant was used for biochemical analysis using a Shimadzu UVvisible spectrophotometer

To achieve quantification and external validation, high-resolution imaging should be paired with mechanical measurements to resolve skin-surface microrelief trajectories and cellECM interaction forces, with standardized QC readouts across barrier function, secretome, and mechanics 131,134

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The expression of SELENOW is correlated with selenium status and appears to be sensitive to low-selenium supply (19, 20)

This review encapsulates recent advancements in research, highlighting novel targets and agents, such as natural compounds, synthetic inhibitors, and clinical trial-stage candidates, all centered on ferroptosis strategies, to offer guidance for future therapeutic developments (Table 3)