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After incubation, the batches were centrifuged at 4000 g for 25 minutes and the pellets were re-suspended in lysis buffer (200 mM NaCl, 50 mM Tris-HCl, 5 mM EDTA, 2 mM benzamidine, 1 mM phenylmethylsulfonylfluoride and 0.5 g/l lysozyme, pH 7.8)

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Mass Spectrometry: Data-dependent MS/MS analysis was performed using a Q Exactive Orbitrap mass spectrometer (Thermo Scientific)

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We excluded patients with history of variceal bleeding or uncontrolled ascites or hepatic encephalopathy