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Quantification was performed by comparing sample peak areas to calibration curves ( 2.7.1.2 Evaluation of antibody effects on CD38 enzymatic activity LP-1 cells or primary MM cells (5 10 4 in 0.5 mL) were incubated (30 min at 37 C) in AIM V serum-free medium with serial dilutions of DARA, ISA or control human IgG1 (10 g/mL-100 g/mL)