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We used the APAP administration rates of 0, 0.5, and 6 mM/h for no, moderate, and excessive administration, respectively

PLC-2 then competes with PI-3K for PIP 2 which it hydrolyzes into IP 3 (inositol 1,4,5-trisphosphate), which ultimately raises intercellular calcium, and diacylglycerol (DAG), which activates portions of the PKC family