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The bacterial cells were grown at 20 C and after 4048 h, the cells were harvested by the centrifugation at 6500 g for 10 min, washed 23 times with phosphate-buffered saline (PBS) buffer (pH 7.2) and the cell pellet was re-suspended in ice cold 20 mM Tris-Cl (pH 7.4)

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In theory, PHH isolated from humanized FRG mice can be used for subsequent in vitro cultures or for transplantation, which greatly expands the donor cells when PHH is limited

Doubts also remain concerning the signals I and II that are generated by SARS-CoV-2 replication to activate inflammasomes in the same cell and whether activation of TLR9 was caused by mtDNA or a direct effect of the virus