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An Afe I restriction site directly upstream of the Strep -tag II, together with the unique Xba I restriction site in front of the whole reading frame, was subsequently used to exchange the entire coding region for sfGFP by the ones for different other POIs (see Supplementary Table S1)

Avoid forcing the water against the powder

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The conclusion was further validated by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and H&E staining (Fig

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