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Cardiolipin can be oxidized by ROS or by the cardiolipincytochrome c complex ( c from cardiolipin might also be triggered by increased cytosolic calcium, which weakens the electrostatic interaction between cytochrome c and cardiolipin and further generates ROS via MPT

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Thus, although one can still generate 10 13 chimeric molecules during a reaction, each of the molecules chosen for further analysis most likely has, for example, only three crossover events