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For the NAC rescue experiment, mice received intraperitoneal administration of 200 mg/kg of body weight NAC (A105422, Aladdin) in phosphate-buffered saline (pH 7.4) every second day for two weeks

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It did not provide a separate standardised assessment of an arginate material

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Because the well water is in the open air, it is easily contaminated by mud, bacteria, mosquitoes, flies, microorganisms, and various aquatic nematodes