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3 were acquired using Titan Krios cryo-TEM (ThermoFisher Scientific) equipped with a Gatan Bioquantum energy filter and a K3 Summit direct detector at an acceleration voltage of 300 kV using SerialEM 66 at a magnification of 19,500, at binning 2, corresponding to a pixel size of 0.9 nm at a defocus of 30 m

Edu staining experiment Place KGN cells on a coverslip in a 24-well plate and culture until confluent

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Google Several months ago I came to Optimized Wellness and met with Amy because my psychiatrist recommended that I try bioidentical hormone replacement therapy