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For AChE activity, after a preincubation period of 10 min at 37 C, 10 l of 10 mM acetylthiocholine iodine (ASChI) and 10 l of 4 mM 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) were added to a 96-well microplate and were diluted with phosphate buffer

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The dosage and frequency of injections will be determined by your doctor based on your specific needs