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CuNPs group: Mice were injected intraperitoneally with 0.2 ml (0.667 mg/kg b.w.) of CuNPs started from the seventh day of the experiment daily for 2 weeks (5 times each week)

Bioreactor temperature during infection was investigated to maximize titer, and a decrease in temperature from 37C to 34C yielded a twothree-fold increase in infectivity

Finally the levels of protein carbonyls, as an oxidative stress marker, were detected

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The increased metabolic activity also helps the body process nutrients more effectively, supporting both fat reduction and muscle recovery