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To limit invasion of pathogens, a balance of ROS-scavenging and ROS-producing pathways is necessary for proper defense mechanism

Given the biological effects of oxygen molecules, although the optimal target levels still remain controversial, unnecessary oxygen administration should be avoided, and exposure to hyperoxemia should be minimized in critically ill patients

To extract bacterial metabolites with an acetonitrile-methanol-water (2:2:1) mixture (ACN-MeOH-water), the bacterial cultures were dried overnight at room temperature under vacuum (GeneVac)

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They also found that 4-HNE production in OA articular tissues contributed to inflammatory responses by upregulating COX-2 expression and limiting the magnitude of transcriptional expression of iNOS [80]