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Since the late 20th century, diverse FBS substitutes, including animal- and human-derived supplements, plant-based hydrolysates, and serum-free or chemically defined media, have emerged, each offering distinct advantages and limitations depending on the target cell types and applications

Determination of blood total, reduced, and oxidized glutathione in pediatric subjects

Sikora E, Bielak-Zmijewska A, Dudkowska M, Krzystyniak A, Mosieniak G, Wesierska M, et al

For the elimination of the spin label excess, the RBCs were washed several times with cold phosphate buffer saline (PBS), until the disappearance of the EPR signal in the supernatant

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