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Before degradation or elongation can take place, FFAs need to be activated to acyl-coenzyme A (-CoA) esters by one of a variety of different chain-length-specific synthetases localized in distinct subcellular compartments [3]

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Imaging was performed with a Zeiss Axio Observer.Z1 microscope with a Yokogawa spinning disk confocal head, Cascade IIL:512 camera, a quad-band notch dichroic mirror (405, 488, 568 and 647 nm) and 405-nm, 491-nm, 561-nm and 640-nm lasers (all 50 mW)