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The identification of a new transcription factor which binds sites in the promoter region of these genes underlined differences in the transcriptional regulation of both species

Protein pellet was resuspended in 1 mL of 6 M guanidine hydrochloride at 37C for 30 min with vortex mixing

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Accumulating in vitro evidence indicates that nuclear HDAC13 are the most potent lysine delactylases, whereas SIRT13 also exhibit substantial delactylation activity toward both L- and D-lactyl modifications