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Consistent with this, intracellular ATP levels were most abundant in BMM (Figs

Malumbres, M

The evolving role of investigative toxicology in the pharmaceutical industry

Our differentiation protocol positions these four timepoints as critical for liver organoid development and maturation: Day 7 represents the hepatoblast differentiation stage, Day 15 marks the early hepatocyte stage, and Day 30 and Day 45 indicate distinct stages of liver organoid maturation towards achieving appropriate liver functions with an increasing population of liver cells

doi: 10.3389/fchem.2025.1698116 Received 03 September 2025 Accepted 15 September 2025 Published 30 September 2025 Volume 13 - 2025 Edited by Ruojun Man, Guangxi Minzu University, China Reviewed by Ramesh Maruthi Chingle, National Institutes of Health (NIH), United States Bing Yang, Nantong University, China Xiaowei Hu, Linyi University, China Updates Copyright 2025 Zhou, Zhang, Lao, Zhou and Zhu