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27 , 805815.e4 (2018)

Animals The study included a sample of 42 male albino Wistar rats, with weights ranging from 180 to 220 g

miRNAs were shown to regulate metabolism-related genes in T1D, an autoimmune disease characterized by persistent hyperglycemia secondarily to pancreatic -cell destruction and insulin deficiency (118, 119)

Redissolve: Always centrifuge tubes before opening.Do not mix by vortex or pipetting

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min