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For measurements of the binding between active-caspase-3/GzmB and GSDME WT/Cys mutant: gradient concentrations of recombinant human active-caspase-3 or granzyme B (0.078, 0.156, 0.3125, 0.625, 1.25, 2.5, 5 M) were incubated with 0.2 M prelabelled recombinant GSDME proteins at a volume ratio of 1:1 for 10 min at room temperature

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