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To examine the incorporation of GSH-derived cysteine into downstream pathways, we used an inverse stable-isotope labelling approach 34,35

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Crystallization The KRAS G13C protein was diluted to 2 mg/mL and incubated with a 3-fold excess of the acryl-bearing nucleotide 7b in 100 mM CHES (pH 9.5), 50 mM NaCl, 1 mM EDTA, 1 mM TCEP, and 5 % glycerol at room temperature

The Hepatocellular Carcinoma Database (HCCDB) includes ten standardized HCC subdatasets (OEP000321, GSE22058, GSE36376, GSE54236, GSE63898, GSE25097, GSE76427, GSE121248, GSE89377, and GSE87639), comprising a total of 1359 HCC samples and 974 adjacent nontumor samples and is also accessible online [27]

Research by Read et al