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Ethical principles of the study ensured compliance with European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13, the Animal Protection Act (official Gazette 135/06), Ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), FELASA recommendations, and recommendations of the Ethics Committee, School of Medicine, University of Zagreb

After a wash step, cells were incubated with fixation/permeabilization concentrate for 30 minutes to fix the surface and permeabilize cells for intracellular staining

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To improve efficacy and reproducibility, future research should focus on the standardization of herbal compounds, optimization of clinical trial design, and adoption of integrative approaches that combine herbal therapies with conventional pharmacological treatments

Time since last drink - Your body can only break down one standard drink per hour, meaning the BAC may still be rising several hours after drinking has stopped because the alcohol takes time to be absorbed