Hold the needle in place for 5 to 10 seconds after injecting, then withdraw

Materials and methods Materials The materials used in this study included fresh mung beans (acquired from a local Sams Club market), PEG8000 (Chron Chemicals), Nrf2, HO-1, P-Akt, Akt, GSK-3, P-GSK-3, GLUT4, Lamin B, -actin (Abclone), Total cell RNA extraction kit (FORE GENE), GN5K DNA Maker (Servicebio, G3361), BCA Protein Concentration Assay Kit (BOSTER,18B06A46), SDS-PAGE protein loading buffer (BOSTER,18C20B12), Color Rapid Gel Preparation Kit 10% (BOSTER,18D11B47), reverse transcription pre-mix kit (Accurate biology, AG11728), Three-color prestained protein standards (Accurate biology, AG11919), DID (cell membrane red fluorescent dye, UElandy, 230510E01-02), BSA (BioFroxx), AST assay kit (mindray, 140,220,009), ALT assay kit (mindray, 140,120,012), SOD assay kit (Nanjing Jiancheng, A001-3-2), Glutathione peroxidase assay kit (Nanjing Jiancheng, A005-1-2), MDA assay kit (Nanjing Jiancheng), Immobilon-p PVDF(Millipore, P2231170), 4% paraformaldehyde universal tissue fixative (Biosharp, China, 23,011,108), enhanced RIPA lysate (BOSTER,18A12B02), DMED/F12(1:1) medium (Gibco, 8,123,177), DMEM medium (Gibco, 8,122,631), and a high-fat diet (containing 60 kcal% fat, Research Diets, D09100310)

So, the question is, might they be correct
Bolger, Narayanasamy Ravi, John V
10.7150/ijbs.78654 47 LvQ.-K.TaoK.-X.YaoX.-Y.PangM.-Z.CaoB.-E.LiuC.-F.et al (2024)