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Nr.: RV-101) were cultivated in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Thermo Fisher Scientific, USA), 100 U/ml penicillin, 100 g/ml streptomycin (Gibco, USA) and 4 mM L-Glutamine (Gibco, USA)

coli is a familiar commensal bacterium that colonizes the gut soon after birth

Although iron-dependent prolyl hydroxylases (PHDs) regulate both isoforms and iron chelation can stabilize HIF-1 and HIF-2 in vitro (74), these observations collectively suggest that the gut may implement additional, tissue-specific mechanisms that bias HIF isoform activity and downstream iron handling

This limitation has stimulated interest in targeted antiferroptotic approaches that aim to interrupt this pathological cycle and promote tissue repair