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Cysteine liberated from the glucys by ChaC family proteins activity was measured by acidic ninhydrinbased cysteine detection method and do not need to be treated by cysgly peptidase (Dug1p)

Such cell death is associated with a burst-like increase in intracellular Ca 2+ after a brief (several minutes) exposure to H 2 O 2 , as a result of the influx of Ca 2+ from the extracellular space via redox-sensitive Ca 2+ channels, such as TRPM2, which are expressed in neurons 59

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