,
doi: 10.1073/pnas.2208506119 67 BuonannoMGBrennerV
Glutathione therapy is evidence-based cellular support that works at the molecular level, and it sits within our complete IV nutritional therapy program
Functional clinicians don't just ask, What pill can we use?they ask life-shaping questions that connect digestion, immunity, environment, and energy
The compounds used are Ferrostain-1 (Fer-1) (S7243, Selleck), Deferoxamine (DFO) (D9533, Sigma), Propidium iodide (PI) (P4170, Sigma), BODIPY581/591 C11 (D3861, ThermoFisher), Lipofectamine 2000 (11668500, Invitrogen), DCFH2-DA (D6883, Sigma), Cystine (30200, Sigma), Homocysteine (Hcy) (H4628, Sigma), Cystathionine (Cta) (C7505, Sigma), S -adenosylmethionine (SAM) (A7007, Sigma), S -adenosylhomocysteine (SAH) (A9384, Sigma), Methionine (Met) (M9625, Sigma), FIDAS-5 (HY-136144, MCE), PF-9366 (S0435, Selleck), Adenosine Dialdehyde (ADOX) (S8608, Selleck), Mitoguazone (MGBG) (HY-15763, MCE), Erastin (HY-15763, MCE), Imidazole Ketone Erastin (IKE) (S8877, Selleck), Mito-TEMPO (HY-112879, MCE), Oligomycin (HY-N6782, MCE), Antimycin A (MS0070, MaoKangBio), Coenzyme Q4 (C921665, Maklin), RSL3 (HY-100218A, MCE), Doxorubicin (DOX) (HY-15142, MCE), Apoptosis Inducer Kit (TNF-/SM-164, C0006, Beyotime), Necroptosis Inducer Kit (TNF-/SM-164/Z-VAD-FMK, C1058, Beyotime), Annexin V-FITC Apoptosis Detection Kit (C1062, Beyotime), LPS (Sigma, L2630), Nigericin (HY-100381, MCE), Methylnitronitrosoguanidine (MNNG) (HY-128612, MCE), alpha-phenyl-tert- N -butylnitrone (PBN, B100838, Aladdin), iFSP1 (HY-136057, MCE), MitoTracker (M7512, Thermo), Total Glutathione Assay Kit (S0052, Beyotime), Cysteine Content Assay Kit (D799571-0050, Sangon Biotech), LDH Cytotoxicity Assay Kit (C0016, Beyotime), CoQ10 Test Kit (PM23627, PERFEMIKER), Mevalonate ELISA Kit (YX-132201M, Sino Best Biological Technology), Tyrosine Assay Kit (ab185435, Abcam), In Situ Cell Death Detection (TUNEL) Kit (11684817910, Roche), S -adenosylmethionine (SAM) ELISA Kit (BY-JZF0361, BYabscience, Nanjing, China), S -adenosylhomocysteine (SAH) ELISA Kit (BY-JZF0362, BYabscience), Mito Stress Test Kits (101706-100, Agilent)
