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However, there are still challenges that need to be addressed, such as the optimization of properties, reliable characterization methods, safety evaluation, nanoparticles that might lead to unintended consequences like nano-pollution and cytotoxicity and regulatory aspects related to nanomedicines targeting skin cancer [176, 179]

Therefore, SH compounds, reducing agents and SH reactive materials should be avoided during the sample preparation

Besides MLH1 , which is often mutated or downregulated in the context of CDDP resistance, 126, 127, 128, 129 these genes include Rho GDP dissociation inhibitor beta ( ARHGDIB ), armadillo repeat containing, X-linked 2 ( ARMCX2 ), collagen, type I, 1 ( COL1A1 ), filamin A ( FLNA ) filamin C ( FLNC ), mesoderm-specific transcript ( MEST ), neurotensin ( NTS ) and proteasome (prosome, macropain) subunit, type, 9 ( PSMB9 ), pointing to the existence of hitherto unexplored mechanisms whereby malignant cells can lose their sensitivity to CDDP