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Thereafter, the TMB substrate was added, and the color was developed in the dark at 37 C for 15 min

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High-LET radiation elicits qualitatively distinct biological responses at low versus high doses ( From DNA Damage to Stable Phenotype: The Role of the DDRcGASSTING Axis A critical question is how a transient physical insult (irradiation) induces stable, heritable changes in the cellular operating system. We propose that complex, clustered DNA double-strand breaks (DSBs) generated by high-LET heavy ions are the initiating trigger ( Beyond Epigenetic and Transcriptional Reprogramming: Genomic Scars as a Foundation for Phenotypic Stability Beyond epigenetic and transcriptional reprogramming, a fundamental mechanism driving the observed stable phenotypes may reside in the permanent genomic alterations elicited by high-LET radiation

The answer lies in the multiple reinforcing loops: The Genetic Loop: Your underlying genetic factors don't change

5B), as well as the anti-inflammatory cytokine IL-10 (Fig