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In endothelial cells (ECs), copper overload disrupts mitochondrial dynamics, increases fission and ROS production, and ultimately leads to cell death [34] In cardiomyocytes, copper promotes hypertrophy and inflammation [35], disrupts fatty acid and lipid metabolism, upregulates autophagy-associated proteins and genes, and impedes calcium ion uptake by the sarcoplasmic reticulum, thereby inhibiting myocardial contractility [36]

Bulun SE, Yilmaz BD, Sison C, et al

It influences satellite cell activation and promotes intracellular pathways associated with protein synthesis and tissue regeneration

3.3 Insulin-like growth factor binding protein 3(IGFBP-3) IGFBP-3 is the most abundant member of the IGFBP family, primarily functioning to regulate IGF bioavailability through direct binding while also modulating cell proliferation and apoptosis via IGF-independent mechanisms