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The cytochrome P450 enzyme CYP1B1, an extrahepatic isoform involved in arachidonic acid metabolism in the brain cortex and cerebellum, is abundantly expressed in brain microvascular endothelial cells, comprising 77% of CYP isoforms in the brain microvasculature [142,143,144,145]

Supported in animals for MOTS-c specifically [2]

Hence, although future kinetic studies might confirm a random mechanism for the rate-limiting first part of monothiol mechanism (iii) in vitro, GSH is probably the first and the non-glutathione disulfide the second substrate under physiological conditions in vivo (Fig

2013;2(6):e000364

Tyrosinase plays a key role in melanin production