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A mendelian randomization study has found that hyperthyroidism can cause hemodynamic changes, which results in cardiovascular diseases represented by atrial fibrillation (AF) (113, 114)

After incubation, the cells were quickly washed with phosphate buffered saline (PBS, 0.1 M, pH 7.4), harvested, centrifuged, and each pellet was dispersed in PBS, and then sonicated

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