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The conventional processing method for scorpion medicinal material involves boiling live scorpions in salt water until their bodies become stiff, after which they are shade-dried (Commission, 2025)

Under stress conditions, active HO-1 in the mitochondria regulates heme availability for mitochondrial cytochromes (Converso et al., 2006)

doi: 10.1080/1061186x.2022.2085729 176 ChiareliRACarvalhoGAMarquesBLMotaLSOliveira-LimaOCGomesRMet al

The parental yeast strain was treated under heat shock at 42C for 15 min, recovered in 5 mM calcium chloride for 5 min after incubation, and eventually plated on a suitable selection medium ( TABLE 2 List of yeast strains and plasmids used in this study