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The most studied nonsputum biomarkers are LAM ( in vivo and their release or secretion in low amounts, these biomarkers tend to be hardly detectable, have low clinical sensitivity, and are non-specific for Mtb, due to similarities with biomarkers of other mycobacteria ( Interestingly, by using the well-established hypoxia-induced dormancy Wayne model, we analyzed the production of MetE protein during the different stages of growth, and we demonstrated the absence of this antigen in the culture supernatants of non-replicating dormant Mtb ( Figure 3A ), as well as the downregulation of metE gene in dormant bacilli ( Figure 3B )

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