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This site is conserved in most, but not all, classes of GSTs suggesting it may play an important functional role

The Cu2+ ion is coordinated by the histidine imidazole nitrogen and the N-terminal amino group of glycine, forming a specific metal-peptide complex

2448 h post-transfection, the transfected cells were identified and collected by fluorescence-activated cell sorting based on GFP fluorescence using a BD FACSAria II sorter (BD Biosciences, San Jose, CA) equipped with a GFP filter (530/30 nm)

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Long-term users report sustained results with continued use, often noting that they've maintained improvements for years when copper peptides remained part of their routine