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The biomass reaction for the healthy and the activated state was adopted from Bordbar et al 16

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doi: 10.4155/fmc.11.118

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The sheared chromatin was incubated with anti-HMGA1 (Abcam, ab252930,1:50) or anti-control IgG (CST, 2729, 1:50) antibodies at 4 C with continuous rotation overnight