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3 Maintaining a near-physiological pH is crucial to reduce pain, irritation, and tissue damage

In addition, arsenic compromises lysosomal biogenesis and destabilizes lysosomal membrane integrity, further reducing autophagic efficiency

After ultracentrifugation supernatant was stored as cytosolic fraction and the brownish membrane pellet was washed with membrane fraction buffer (5 mL) and centrifuged again