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The total cell lysate was cleared by centrifugation and supplemented with 25 L per liter culture OD 600 of BioLock avidin (IBA Lifesciences, Gttingen, Germany) to mask D-biotin from the culture medium and/or endogenous biotinylated proteins from the host cell, followed by sterile-filtration

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It is naturally produced by the liver and comprises the amino acids cysteine, glycine, and glutamine

Multiple sites on SARS-CoV-2 spike protein are susceptible to proteolysis by cathepsins B, K, L, S, and V

Grover, A.) 113133 (Springer, Singapore, 2017)