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LC-FL analysis Samples derivatized with monobromobimane (mBB) (Life Technologies) were separated with a Waters Symmetry C18 (ID, 250 4.6 mm) column (Waters Corp., Milford, MA, USA) with mobile phase A(0.25% acetic acid in H 2 O, pH 3.9), B(0.25% acetic acid: methanol = 7:3) and C(0.25% acetic acid: methanol = 1:1) with a linear gradient from A:B = 65:35 to 2:8 in 8 min with a flow rate of 1.5 ml/min, and remained with 100% B for additional 10 min, and then changed to 100% C in the following 6 min with a flow rate of 1.0 ml/min

Final action: After consideration of the comments received, we are finalizing at new 180.50(b)(2)(ii)(C)( 2 ) that if the payer-specific negotiated charge is based on a percentage or algorithm, the hospital must calculate and encode the total number of allowed amount remittances from the EDI 835 ERA transaction data or an alternative, equivalent source of remittance data used to calculate the median, 10th percentile, and 90th percentile allowed amounts