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Following 3 hours of feeding the CR mice at the start of the light cycle, all dietary groups underwent a 3 hr fast, followed by cervical dislocation and tissues for molecular analysis were flash-frozen in liquid nitrogen or fixed and prepared as described in the methods below

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The N-terminal region of acyl-CoA synthetase 3 is essential for both the localization on lipid droplets and the function in fatty acid uptake

Clinical evaluation Participants underwent a thorough psychiatric evaluation by trained psychologists using the K-SADS [53] during the baseline evaluation (V0)