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To compare simulated concentration time-courses with measured time-course data, we include the unknown concentration scale of fluorescence microscopy measurements and possible background fluorescence into an observation function

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doi: 10.1038/nature12726 220 FurusawaYObataYFukudaSEndoTANakatoGTakahashiDet al

Therefore, high GSH/GSSG ratios in cancer cells can also be explained by increased flux through the pentose phosphate pathway (PPP), which produces NADPH (Li et al., 2014a

Some practitioners combine both approaches