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Protocol: 116/17)

The cell pellet was suspended in 10% ice-cold TCA and centrifuged for 15 min at 9,000 g

Mitochondria-associated programmed cell death as a therapeutic target for age-related disease

Hepatocytes were transfected with 2.75 M dsiRNA (Integrated DNA Technologies, Coralville, IA) in nuclease-free duplex buffer using the Viromer Blue transfection kit (Origene) following the manufacturers protocol

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