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The supernatant containing His-tagged histones was then incubated with NiNTA resin (Complete His-Tag Purification Resin, Roche) equilibrated with buffer B (50 mM Tris-HCl pH 8.0, 500 mM NaCl, 6 M urea, 5 mM imidazole, and 5% glycerol) for 1 h with end-over-end mixing at 4 C

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In addition, although our mechanistic gain- and loss-of-function studies were supported by extensive in vivo validation, part of the functional characterization was performed using HL60-derived neutrophil cells

Molecular Formula: C38H49N9O5 Sequence: H-Aib-Ala(Unk)-D-2Nal-D-Phe-Lys-NH2 Molecular Weight: 711.9 g/mol Source: PubChem Buy Ipamorelin Peptide Vial Hungary online here Available in 2mg & 5mg