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doi: 10.1074/jbc.M601876200

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They considered that riboflavin deficiency interfered with iron utilization but not absorption 46

For mitochondrial activity, the cells were incubated with 200 mM MitoTracker Deep Red (Thermo Fisher Scientific, Barcelona, Spain) for 20 min at 38.5 C in the dark and then stained with 10 M YO-PRO-1 and 0.5 M PI