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Erythrocytes are stained with Giemsa stain (Sigma-Aldrich) according to Giemsas Staining Protocol

Given that increased inflammation is believed to play an important role in thyroid dysfunction, selenium as an anti-inflammatory substance mediated by selenoproteins could improve thyroid function, which might be propitious to regulate the synthesis and secretion of thyroid hormones (10)

However, phosphorylated AKT (p-AKT) and phosphorylated p38 (p-p38), the activated forms of AKT and p38, remarkably decreased in HCT116 GSTA4 cells compared with HCT116 cells, indicating inhibition of AKT and p38 signaling pathways

Allocation sequence will be determined by sequence in permuted blocks of four

However, due to the limited sample size and lack of long-term follow-up in existing studies, we lack an in-depth understanding of the impact and incidence of this complication