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PGI2 inhibits platelet aggregation by activating platelet IP receptors, which couple to Gs proteins and increase intracellular cAMP

Briefly, similar to the MTT assay, glioblastoma cells (5 10 3 ) were seeded in 96-well plates (Kasvi ) and incubated at 37 C for 48 h to allow adequate adhesion to the plates (confluence between 70% and 90%)