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One fraction for analysis of GSH-derived metabolites was immediately precipitated with MeOH and after evaporation, obtained residue was resuspended in 50 L of 50 mM HEPES (pH 7.4), diluted with 20% ACN (containing 0.1% FA and mixture of internal standards) and immediately analysed by LC-MS/MS

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The concentration of glutathione in the cell is 10mM (BNID 104679, 104704, 111464), making it the second most abundant metabolite in the cell (after glutamate) ensuring that it plays a dominant role as an electron donor in redox control of protein function

Drug discovery and screening : In pharmaceutical research environments, Dihexa is incorporated into high-throughput screening assays and lead optimization protocols

Spermidine Alleviates Experimental Autoimmune Encephalomyelitis Through Inducing Inhibitory Macrophages