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Elevated Hcy leads to an increase in the generation of mitochondrial ROS, resulting in oxidative damage at the cellular and molecular levels, and suppression of antioxidant enzyme systems (Kaplan et al., 2020)

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Continuing into the duodenum, pancreatic proteases act on the Hc- Cbl complex

Supplementary Validation Method: LCMS/MS For high-sensitivity detection requirements, liquid chromatographytandem mass spectrometry is used for supplementary validation, especially suitable for trace component analysis and metabolic studies