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10.1016/j.jnutbio.2020.108515 3 ChenJ.LiuQ.HeJ.LiY

J Clin Endocrinol Metab 84 : 39563960 Nasu M et al

2-10 nmol of L-glutamate standards and the cell lysate were mixed with reaction buffer (final concentrations of 36.8 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH 7.1), 2.1910-5 U L-glutamate oxidase, 1 U peroxidase, 0.8 mM 3.5-dimethoxy-N-ethyl-N-(2-hydroxy-3-sulphopropyl) aniline (sodium salt) (DAOS), 0.8 mM aminoantipyrine)