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A transfection mix was prepared with 17.2 l plain DMEM (Gibco) and 0.4 l DharmaFECT 1 transfection reagent (Horizon Discovery, T-2005-01) per well

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But even in meat-eaters, conditions affecting the stomach (like gastritis) or the end of the small intestine can block its absorption, leading to anemia and serious neurological problems (like tingling, memory fog, and balance issues)