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Wound Repair and Regeneration 17:153

Abstract Once considered the exclusive property of conjugated molecules, efficient and visible-light luminescence from non-conjugated and nonemissive molecules in the clustered state, known as clusteroluminescence (CL), has attracted much attention recently due to its special photophysical behaviors and behind electronic interactions

To each well was added 50 L of lysate supernatant, 50 L of milli-Q H 2 O and then 100 L of GA buffer (NADPH 400 M, glutathione reductase 1 U/mL and 0.3 mM DTNB in KPE buffer diluent)

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To further establish that R4VPL3-1 induced ferroptotic cell death and test for potential involvement of additional cell death pathways that may mediate R4VPL3-1-induced cell death, we pre-treated A375R melanoma cells either with DMSO (control), or with Z-VAD-FMK (pan-caspase inhibitor), chloroquine (lysosomal inhibitor), or Necrostatin (a necroptosis inhibitor), or Ferr-1 (ferroptosis inhibitor), and subsequently treated the cells with 3M R4VPL3-1